Journal: Journal of Histochemistry and Cytochemistry
Article Title: Expression of FAM159B in Humans, Rats, and Mice: A Cross-species Examination
doi: 10.1369/00221554241262368
Figure Lengend Snippet: Double-labeling immunohistochemical analysis of FAM159B and somatostatin-14/28 (SST) expression in Langerhans islets from lean and obese control and ciprofibrate-treated Zucker rats. Labeling for FAM159B was visualized using Cy3-conjugated anti-rabbit antibody (red; A, D, G, J) and for SST using Alexa Fluor-488–conjugated anti-rat antibody (green; B, E, H, K); overlapping expression is shown in orange/yellow color (C, F, I, L). Scale bar: 100 µm.
Article Snippet: For double-labeling experiments, samples were incubated overnight at 4°C with anti-FAM159B HPA011778 antibody together with monoclonal mouse anti-insulin antibody (1:100; Abcam, Cambridge, MA), monoclonal mouse anti-glucagon antibody (1:500; Sigma-Aldrich, St. Louis, MO), or monoclonal rat anti-SST-14/28 antibody (1:300; Abcam).
Techniques: Labeling, Immunohistochemical staining, Expressing, Control